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class="clear"></div> </div> <div id="1xx5uidq" class="blockindex h450"> <div id="1xx5uidq" class="new9"> <div id="1xx5uidq" class="new91"> <h1>離子色譜如何選擇適當?shù)牧芟匆?/h1> 發(fā)布時間：2024-09-08 </div> <div id="1xx5uidq" class="new92"> <div class="1xx5uidq" id="contentt"></div> 離子色譜分離是基于淋洗離子和樣品離子之間對樹脂有效交換容量的競爭，為了得到有效的競爭，樣品離子和淋洗離子應(yīng)有相近的親合力。下面舉例說明選擇淋洗液的一般原則。用co2-32hco-作淋洗液時，在cl-之前洗脫的離子是弱保留離子，包括一價無機陰離子、短碳鏈一元羧酸和一些弱離解的組分，如f-，甲酸，aso-2，cn-和s2-等。對乙酸，甲酸與f-、cl-等的分離應(yīng)選用較弱的淋洗離子，常用的弱淋洗離子有hco-3，oh-和b4o2-7。由于hco-3和oh-易吸收空氣中co2，co2在堿性溶液中會轉(zhuǎn)變成co2-3，co2-3之淋洗強度較hco-3和oh-大，因而不利于上述弱保留離子的分離。b4o2-7亦為弱淋洗離子，但溶液穩(wěn)定，是分離弱保留離子的*淋洗液。中等強度的碳酸鹽淋洗液對高親和力組分的洗脫效率低。對離子交換樹脂親合力強的離子有兩種情況，一種是離子的電荷數(shù)大，如po3-4，aso3-4和多聚磷酸鹽等；一種是離子半徑較大，疏水性強，如i-，scn-，s2o2-3，苯甲酸和檸檬酸等。對前者以增加淋洗液的濃度或選擇強的淋洗離子為主。對后一種情況，*的方法是在淋洗液中加入有機改進劑（如甲醇、乙腈和對氰酚等）或選用親水性的柱子，有機改進劑的作用主要是減少樣品離子與離子交換樹脂之間的非離子交換作用，占據(jù)樹脂的疏水性位置，減少疏水性離子在樹脂上的吸附，從而縮短保留時間，減少峰的拖尾，并增加測定靈敏度。<br>在離子色譜中，可由加入不同的淋洗液添加劑來改善選擇性，這種淋洗液添加劑只影響樹脂和所測離子之間的相互作用，而不影響離子交換。對與樹脂親合力較強的離子，如一些可極化的離子，i-和clo4-，以及疏水性的離子，苯甲酸和三乙胺等，在淋洗液中加入適量極性的有機溶劑如甲醇或乙腈，可縮短這些組分的保留時間并改善峰形的不對稱性。為了減少樣品離子與樹脂之間的非離子交換作用，減少樹脂對疏水性離子的吸附，在陰離子分析中，可在淋洗液中加入對氰酚。如測定1%nacl中的痕量i-和scn-時，加入對氰酚占據(jù)樹脂對i-和scn-的吸附位置，從而減少峰的拖尾并增加測定靈敏度。ic中，一價淋洗離子洗脫一價待測離子，二價淋洗離子洗脫二價待測離子，淋洗液濃度的改變對二價和多價待測離子保留時間的影響大于一價待測離子。若多價離子的保留時間太長，增加淋洗液的濃度是較好的方法。<br> 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