來源于escherichia coli（e.coli）的片段dna可以用作核酸酶靶向切割和釋放（cut&run）的實驗標準化的spike-in對照。產品cutana™ e. coli spike-in dna含有足夠的材料，可用于100-200個cut&run樣本（高豐度和低豐度靶標的范圍）。
產品詳情
保存溫度:stable for 2 years at -20°c from date of receipt. after resuspending, aliquots should be stored at -20°c.
運輸溫度:room temp. can go on frozen cold packs or dry ice.
產品形式:100 ng lyophilized dna. *note: may not be visible.
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驗證數(shù)據(jù)
figure 1: dna gel data
e. coli fragment size distribution. lane 1: gdna extracted from jm101 e.
coli cells (500 ng). lane 2: digested and purified cutana™ e. coli spike-in
dna (500 ng) resolved via 2% e-gel™ ex agarose gel. migration positions
of dna molecular weight markers are indicated.
figure 2: cut&run sequencing data
cutana™ e. coli spike-in dna (1.0 ng) was added to cut&run samples using 500,000 k562 cells enriched
for a low abundance target (h3k4me3, epicypher 13-0041), a high abundance target (h3k27me3, epicypher
13-0030) and an igg negative control (epicypher 13-0042). total numbers of paired-end sequencing reads, reads
aligned to e. coli, and percentage of total sequencing reads aligned to e. coli spike-in dna are shown. note:
spike-in dna amount should be optimized by the end user with the goal of e. coli dna comprising ~1% (0.2 -
5%) of total sequencing reads.
訂購詳情
貨號
產品名稱
規(guī)格
18-1401
cutana™ e. coli spike-in dna
100 ng
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