lc-ms/ms法測(cè)定血漿中的原霉素組分分析pristinamycin components in plasma by lc-ms/ms
ace 3 c18 3μm, 30 x 3.0mm
gradient analysis
a = 1mm nh4co2h + 0.1%
hco2h in 65:35 h2o:ch3cn
b = ch3cn
t (mins) %b t (mins) %b
0 0 1.61 100
0.3 0 2.6 100
0.31 10 2.61 0
1.6 10 4 0
flow rate: 1ml/min
column temperature: 25°c
injection volume: 10μl
mds sciex api 4000
turboionspray positive mode
transitions monitored:
pristinamycin la 867.5 → 134.2
pristinamycin lla 526.3 → 355.1
i.s. (virginiamycin) 824.6 → 134.0
pristinamycin antibiotic is a mixture of 2 components –pristinamycin la and lla
virginiamycin used as internal standard