*、*，hplc熒光檢測tyrosine, tryptophan and tramadol by hplc with fluorescence detection
conditions
column:ace 5 c18
dimensions: 150 x 4.6 mm
part number: ace-121-1546
mobile phase: a: 50 mm nah2po4 in h2o
b: mecn/h2o (60:40 v/v)
gradient:
time(mins)
%b
0.0
0
5.0
10
8.0
100
9.0
100
9.5
0
14.0
0
flow rate: 1 ml/min
injection: 5 μl
temperature: 25 °c
detection: fluorescence, λex 280 nm, λem 340 nm
analytes
1. l -tyrosine
2. l -tryptophan
3. tramadol