rapid detection of e. coli on goat meat by electronic nose
電子鼻技術(shù)檢測(cè)山羊肉中大腸桿菌的研究
ding ning-ye1
lan yu-bin2
zheng xian-zhe3
abstract:much attention has been paid on the foodborne illness of food, which is easily contaminated with bacteria or pathogens. escherichia coli (e.coli) is one of these bacteria that commonly live in the contaminated animal meat. there is a growing need in the food industry for pathogen detection systems that are sensitive to low levels of bacteria, specific to the target organisms, capable of yielding results at or near real time. both contaminated and non-contaminated goat meat were tested using an electronic nose (cyranose 320) which consists of 32 polymer sensors. we developed an electronic nose method for the rapid detection of e. coli o157:h7 in goat meat. principal component analysis (pca) method was applied to analyze the experimental data, and the results indicated that they either overlap or are very close making it very difficult for the device to correctly identify. e-nose has a potential for being used as a tool for rapid detection of contamination, although it is not able to detect very low concentration of the contaminant.
文摘：食品中易受細(xì)菌或病原體污染的食源性疾病引起了人們的廣泛關(guān)注。大腸桿菌(e.coli)是這些細(xì)菌中的一種，通常生活在受污染的動(dòng)物肉類中。食品工業(yè)越來(lái)越需要對(duì)低水平的細(xì)菌敏感的病原體檢測(cè)系統(tǒng)，這些細(xì)菌是針對(duì)目標(biāo)生物體的，能夠?qū)崟r(shí)或近實(shí)時(shí)地產(chǎn)生結(jié)果。使用由32個(gè)聚合物傳感器組成的電子鼻(cyranose320)對(duì)受污染和未受污染的山羊肉進(jìn)行測(cè)試。我們開發(fā)了一種快速檢測(cè)山羊肉中大腸桿菌o157:h7的電子鼻法。采用主成分分析(pca)方法對(duì)實(shí)驗(yàn)數(shù)據(jù)進(jìn)行了分析，結(jié)果表明，這些數(shù)據(jù)要么重疊，要么非常接近，使得裝置很難正確識(shí)別。電子鼻有潛力被用作快速檢測(cè)污染物的工具，盡管它不能檢測(cè)到非常低濃度的污染物。
keywords:goat meat; bacteria (e.coli); electronic nose; quality detection